AAB Molecular Diagnostics Practice Test 2026 - Free Molecular Diagnostics Practice Questions and Study Guide

The primary function of the nylon membrane in the Southern Blot process is related to its role in hybridization of the labeled DNA. After DNA fragments are separated by gel electrophoresis, they need to be transferred to a solid support for further analysis. The nylon membrane serves as this solid support, providing a stable surface for the DNA fragments to adhere to.

Once the DNA is transferred onto the nylon membrane, the membrane can then be treated with labeled probes that hybridize to specific sequences of interest. This allows researchers to visualize and identify the presence of particular DNA sequences within the complex mixture obtained from the gel. The nylon membrane's ability to bind DNA efficiently makes it an ideal medium for this crucial step in Southern Blotting.

The other options do not accurately describe the function of the nylon membrane in this context. Amplification, transfer of RNA samples, and facilitating gel electrophoresis pertain to different processes or techniques outside the specific role of the nylon membrane in the Southern Blot methodology.